Comparative Effectiveness of Defatted Hypotensive Crude Extract, Ethyl Acetate and Butanolic Fractions of Cassytha filiformis l. on Different Models of Hypertensive Rats

Armenia, Y. Yuliandra and M. Z. A. Sattar

The hypotensive effectiveness of defatted crude ethanolic extract, ethyl acetate and butanolic fractions of Cassytha filiformis L. have been compared on anesthesized prednisone-saline-induced (PN) and Prednisone-saline-LNAME -induced (PNL) hypertensive rats. Extract/fractions were given at the dose of 5 mg/kg each while a group treated by 100 μmol/kg oftempol was used as comparison. Extract/fractions/vehicle were commence in 3 consecutive doses intravenously every one hour interval. The systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and heart rate (HR) of the animals were measured (Biopac® MP 150 Data Acquisition System). Data were presented as the percentage changes of those parameters and analyzed by three way ANOVA followed by Duncan’s Multiple Range Test. Results showed that the ethanolic defatted extract and tempol decreased animal SBP, DBP, MAP and HR significantly (p<0.05) while ethyl acetate and butanolic fractions did not (p>0.1). The average percentage decrease of animal SBP and MAP on PNL rats were higher (p<0.05) as compared to those on PN rats, while DBP and HR of those groups of animal were not significantly different (p>0.1). Repeated dose of all samples tent to decrease animal SBP and HR (p<0.1) but not their DBP and MAP (p>0.1). These results indicated that the hypotensive effectiveness of the defatted ethanolic extract of Cassytha filiformis is better as compared to its ethyl acetate and butanolic fractions and these effects are greater on oxidative stress related hypertensive rats.

Keywords: hypotensive activity, prednisone-saline induced, prednisone-saline-L-NAME induced, Cassytha filiformis.

Published in: World Journal of Pharmacy and Pharmaceutical Sciences, Volume 3, Issue 12, 200-208.

Daun Tanaman Akar Mambu (Connarus grandis Jack.) Sebagai Obat Anti Hipertensi: Efektivitas Ekstrak Etanolnya pada Tikus Hipertensi 2K1C Goldblatt

Armenia, Welmidayani, Y. Yuliandra dan Rusdi

Jurusan Farmasi FMIPA Universitas Andalas, Padang

ABSTRACT

An antihypertensive effect of Connarus grandis Jack leaves ethanolic extract has been investigated on anesthetized two kidney one clipt (2K1C) Goldblatt hypertensive rats. Four groups of animals were treated with the extract at doses of 10, 20, 40 and 80 mg/kg BW intra peritoneally. These doses were repeated every half an hour. Blood pressure (systolic, diastolic, mean) and heart rat were measured ten miknutes after each dose. As comparisons, a group of control animals (treated with saline) and another treated with Captopril (2.5 mg/kg) were

Monitoring Efektivitas Terapi dan Efek-Efek Tidak Diinginkan dari Pengobatan Hipertensi Menggunakan Diuretik dan Kombinasinya di Poliklinik Khusus RSUP Dr. M. Djamil Padang

Yuanita Ardi*, Armenia*, Masrul Syafri**

*Faculty of Pharmacy, Universitas of Andalas, Padang-Indonesia
**Faculty of Medicine, Universitas 0f Andalas, Padang-Indonesia

ABSTRACT

Most of the hypertension therapy demonstrate uncontrolled blood pressure. One of the most prescribed drug for this patients is diuretic (either single or combination), which cause electrolyte disturbances. The aim of this study is to evaluate the effectiveness of therapy and adverse drug reaction of diuretic (HCT) and its combination in hypertensive patient at special clinic of RSUP Dr. M. Djamil Padang. This prospective observational study involve 20 patient which were divided into five groups based on antihypertensive drugs are prescribed, namely group I (only HCT), group II (HCT and 1 other antihypertensive), group III (HCT and 2 other antihypertensives), group IV (1 non-diuretic antihypertensive), and group V (2 non-diuretic antihypertensive). Blood pressure and heart rate of patients are measured on days 2, 5, 7, 10, and the

Efek Penurunan Tekanan Darah dan Gula Darah dari Fraksi Air Daun Sambung Nyawa (Gynura procumbens) Terhadap Tikus Hipertensi Diabetes

Helmice Afriyeni, Armenia, Deddi Prima Putra

ABSTRAK

Hipertensi sering menyertai peyakit diabetes mellitus. Hal ini meningkatkan morbiditas dan mortalitas karena meningkatkan resiko terjadinya penyakit jantung koroner, stroke, nefropati dan retinopati. Penelitian ini bertujuan untuk mempelajari efek penurunan tekanan darah dan gula darah dari fraksi air daun sambung nyawa (Gynuraprocumbens. Lour. Merr.) pada tikus hipertensi-diabetes. Tikus wistar diinduksi hipertensi dengan kombinasi prednisone 1,5 mg/kgBB dan NaCl 2,5% peroral selama dua minggu, sedang diabetes diinduksi menggunakan aloxan monohidrat 150 mg/kgBB diberikan secara intra peritoneal. Hewan-hewan yang sudah hipertensi dan diabetes dikelompokkan menjadi 4 kelompok, satu kelompok sebagai kontrol yang hanya diberi larutan NaCl fisiologis, sedangkan 3 kelompok lainnya diberi fraksi air daun sambung nyawa dengan dosis berturut-turut 10, 20 dan 40 mg/kgBB secara intra vena dengan pengulangan tiga kali. Percobaan dilakukan pada tikus teranestesi (pentobarbital Na,

Akar Mambu (Connarus grandis Jack.) and its antihypertensive effect

Yori Yuliandra, Armenia, Rusdi

ABSTRACT

The effect of the aethanolic leaf extract of Connarus grandis Jack. to the blood pressure of hypertensive rats was investigated. An amount of 2 to 3 months old male Wistar Kyoto rats weighing 200 – 300 g were hypertensive-induced with prednisone 1,5 mg/kg and NaCl 2,5 % orally each day. After two weeks of hypertensive-induction treatment, 18 rats were grouped into 6: hypertensive control group, standard drug-treated group (nifedipine 5 mg/kg), and extract-treated group (extract 10, 20, 40, and 80 mg/kg). The rats were anesthetized with pentobarbital Na 60 mg/kg and then were cannulated at the carotid artery to perform direct blood pressure measurement. The Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), Mean Arterial Pressure (MAP), and Heart Rate (HR) were recorded by using